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HuSIGHT™

Human Protein Antibody Reactome Profiler

Obtain a deep understanding of how autoimmune responses impact health and disease states.

What Does HuSIGHT Cover?

HuSIGHT is the world’s only combined full-length protein and peptide tile library:
27,703
unique genes covered
385,432
unique protein isoforms tiled by peptides

What Does HuSIGHT Contain?

16,516
unique full-length proteins
353,034
unique and overlapping ~87 amino acid long peptides
880
full-length mature secreted protein isoforms

Benefits of Detecting Autoantibodies

The unbiased detection of autoantibodies can:

  • Provide new insights into disease mechanisms
  • Discover novel therapeutic targets
  • Lead to the development of new drugs and diagnostic tests

It has also become clear that autoantibodies can harbor both beneficial and harmful activities, and their presence or absence can be strongly associated with divergent health outcomes.

By studying autoantibodies, researchers can gain a much more detailed understanding of how immune responses impact health and disease states.

autoantibodies

How HuSIGHT Works

There are complementary benefits of unbiased autoantibody profiling with peptide libraries versus full-length protein libraries.

Peptide libraries can be very comprehensive in their coverage of all human gene products and their alternatively spliced variants. However, many autoantibodies only recognize the conformational or discontinuous epitopes provided by the 3D folding of full-length proteins.

This type of autoantibody would be undetectable with an assay composed only of peptides. On the other hand, autoantibody detection with an assay composed only of full-length proteins, would be unable to detect the antibodies that target proteins that are difficult to express or simply missing from the library. And in some cases, the importance of an autoantibody is determined not simply by whether the full-length protein is targeted, but rather which specific region or epitope of the protein is targeted.

For these reasons, Infinity Bio has created the first autoantibody profiling assay that combines a comprehensive human peptide library with a large set of full-length human proteins, all in a single, cost-effective assay.

The HuSIGHT-Full_Length sublibrary was constructed from ThermoFisher’s sequence-verified Ultimate ORFeome, which contains 16,516 unique proteins. Request a copy of the HuSIGHT-Full_Length sublibrary.

The HuSIGHT-peptide sublibrary was designed using Infinity Bio’s proprietary approach to multiple sequence alignment-based tiling of all human protein sequences and their variations which are present in the UniProt and RefSeq databases. Request a copy of the composition of the HuSIGHT-peptide sublibrary.

These overlapping peptide tiles are ~87 amino acid long with ~30 amino acid overlaps, allowing flexibility in length to ensure optimal comparability across homologous regions of a clustered sequence alignment. The optimized human peptidome library design parameters incorporate our decade-plus of experience performing autoantibody profiling with peptide libraries.

”"Infinity bio attracted our interest since we for a long time had plans to search for autoantibodies and viral antibodies in our large collections of samples and this company can now help us with state-of-the art technology, which is great.”

“We are excited to test the novel technology provided by Infinity Bio to test whether this can advance our research into new avenues.”

“I am impressed by the speed and excellent service provided by Infinity Bio, the set-up of the experiment, logistics and analyses have worked very well so far."

Peter Bergman, MD. PhD.Professor, Karolinska Institute

HuSIGHT Human Protein Antibody Reactome Profiler Services FAQs

Is my human protein of interest present in the library?

Yes, by combining our human full-length and peptide sublibraries, all proteins are represented, with most represented as both peptide tiles and full-length proteins.

Contact us to see the composition of the HuSIGHT-Full_Length sublibrary or the HuSIGHT-peptide sublibrary.

How does HuSIGHT compare to phage-based assays?

Phage-display methods are limited to peptide libraries and are therefore unable to detect conformational autoantibodies.

HuSIGHT leverages the unique advantages of MIPSA (Molecular Indexing of Proteins by Self Assembly) to create sublibraries that combine peptide and full-length representation of the human proteome. The VeraCode ™ system for multiplexed barcoding of each antigen provides additional protection from the higher rate of false-positive detection associated with phage display-based methods.

How does HuSIGHT compare to protein arrays?

Protein arrays suffer from the many artifacts associated with the staining and imaging of solid supports as compared to solution-phase assays.

Fluorescence detection methods tend to suffer from a more limited dynamic range compared to DNA sequencing-based technologies.

Full proteome arrays are also typically very costly, often resulting in underpowered studies that report false positive associations or missed subgroup responses. MIPSA provides a cost-effective solution, enabling a unique combination of full-length and peptide sublibraries.

Related Analysis Options

If you are interested in the interplay between autoantibodies and environmental exposures, you can also cost-effectively combine HuSIGHT with VirSIGHT and/or AllerSIGHT analyses. Combining these libraries might reveal the impact of certain autoantibodies, such as those targeting the interferon viral response system for example, in modulating the clinical course of a viral infection or allergen exposure.

Find Out How Our HuSIGHT Human Protein Antibody Reactome Profiler Services Can Help You

In addition to standard IgG profiling, we offer profiling of IgA autoantibodies (related to mucosal immunity), IgM autoantibodies (indicative of a naïve reactivity or recent response), and IgE autoantibodies (related to allergic responses).

Contact us to start your project.

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