Specialized Services

Custom Antigen Libraries

Design your custom antigen library to cover any protein set from any organism, including bacteria, fungi, parasites, plants, and viruses.

Build a Custom Antigen Library For Your Research Needs

From epitope to protein-level antigen libraries, we offer an infinity of antibody profiling possibilities.

Design and build sequence-verified antigen libraries with our novel Molecular Indexing of Proteins by Self-Assembly (MIPSA) technology for:

Unparalleled flexibility
Rapid turnaround
Cost efficiency

Our proprietary library assembly process ensures highly uniform libraries with negligible dropout, resulting in maximal sensitivity to detect antibody interactions across each precious member of your library.

How It Works

We typically associate ~100 unique barcodes with each library member to optimize both assay robustness and mapping rate.

We use VeraCode™ technology during our library dictionary construction process to:

Determine those barcodes associated with unmutated library members
Filter your results to these unmutated library members
Ensure your data is uncontaminated by mutant antigens and thus entirely interpretable

Three Protein Representation Methods

We provide three different methods for representing sets of proteins using peptides encoded by synthetic oligonucleotides of up to 90 amino acids long.

Simple tiling:

You may select this simple approach to each protein using constant-length peptides of your preferred length and overlap.

Cluster:

You can select to cluster your protein database using multiple sequence alignment (MSA), followed by MSA-based tiling with variable length peptides of your preferred length and overlap.

Proteome sketching:

You can also have us subsample your protein database using epitope prioritization and optional concatenation of shorter peptides to achieve maximal library compression.

Custom MIPSA libraries can also be constructed for deep mutational scanning of important epitopes. Applications include:

Fine mapping of antigen-antibody interactions
Off-target prediction
Analysis of phylogenetic breadth of coverage

”"We were looking at ways to interrogate the full antibody repertoire of children following measles infection versus controls who have not had measles. We were aware of the work with PhIP-Seq originally in measles and the MIPSA is an advancement on this technology. We also liked that we could add custom libraries to fit our purposes (endemic pathogens in select regions in Africa). The ability to use small volumes of specimens and assistance with all the analysis were also attractive to us. This will help us with speed in publishing and disseminating the results. Infinity Bio has been helpful at each step for us from the science to the business"
Lead Scientist NIAID(Disclaimer: this is a personal statement and not an official statement of NIAID)

Custom Antigen Library FAQs

How long does it take to make a custom library and what is the cost?

Once peptide design is completed, your custom antigen library can be assembled and functionally tested in 4 to 6 weeks. The cost of custom libraries varies due to differences in peptide lengths and overall library complexity.

Is there a size limit for custom libraries?

Our current peptide library size limit is 1,000,000 members. However, please inquire if your library needs exceed this limit, as we may be able to accommodate.

Can I provide my cloned full-length protein coding sequences for incorporation into a MIPSA library?

Most likely. If your previously cloned ORFs are readily compatible with our existing MIPSA library construction, we can incorporate them. Alternatively, we can discuss adaptation or using gene synthesis to recreate them in a compatible format.

Let Us Know About Your Specific Project Goals!

We offer an infinity of possibilities for custom libraries that contain protein domains or full-length proteins.

Our technical team can also help with sourcing your coding sequences, and if they are not commercially available, our gene synthesis partners can ensure that those needs are met, too.

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